Method for screening personalized intestinal environment-improving material and composition therefor
AssigneeHEM, Inc.
InventorsSoyoung Park, Yosep Ji
Life of this patent
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Abstract
The present disclosure relates to a composition for screening an intestinal environment-improving material and a screening method using the composition, and according to the composition and the method of the present disclosure, it is possible to provide an effective analysis method for screening a microbiota-improving candidate material in a personalized manner by providing a method for verifying personalized probiotics, prebiotics, foods, health functional foods and drugs under in vitro conditions based on microbiota and microbiota metabolites.
Claims13
A method for screening an intestinal environment-improving material, comprising:
- (a) mixing a composition for screening an intestinal environment-improving material with a sample obtained from a subject;
- (b) treating the mixture from step (a) with one or more intestinal environment-improving candidate materials and culturing the mixture;
- and
- (c) analyzing the culture from step (b) to analyze toxin, intestinal microbiota, microbiota-derived metabolite, or a mixture thereof in the culture to determine content or concentration thereof,
- wherein
- the intestinal environment-improving material is selected from the group consisting of probiotic, prebiotic, food, health functional food, drug and a mixture thereof;
- the composition for screening an intestinal environment-improving material does not include protein and carbohydrate;
- and
- the toxin is endotoxin or hydrogen sulfide.
A method for screening an intestinal environment-improving material, comprising:
- (1) preparing a mixture of human or animal feces and a Pharmaceutical Meta-Analysis Screening (PMAS) medium by the steps of:
- (a) obtaining feces of a human or animal and mixing the feces with a PMAS medium,
- (b) homogenizing the mixture using a stomacher;
- (c) filtering fecal residues from the feces;
- (d) reducing the feces-medium mixture in an anaerobic chamber for 4 hours,
- (2) Dispensing identical quantities of the feces-PMAS medium mixture into culture plates in an anaerobic chamber;
- (3) screening each candidate intestinal environment-improving material by the steps of:
- (i) suspending each of the candidate materials in sterile 1×PBS;
- (ii) homogenizing each of the candidate materials and then dispensing the homogenized candidate materials to the respective plates prepared in Step (2) where the feces-medium mixture was placed,
- (4) culturing the prepared plates under anaerobic conditions with temperature, humidity and motion similar to those of the intestinal environment to ferment and culture the respective test groups;
- and
- (5) centrifuging the culture products of step (4) to obtain supernatant;
- and
- (6) testing for the presence of toxic or undesirable substances comprising hydrogen sulfide, bacterial LPS, short-chain fatty acids in the supernatant, and/or undesirable microbiota;
- and identifying those intestinal environment-improving material wherein the corresponding cultured sample results in reduced levels of toxic or undesirable substances relative to a control fecal sample not treated with a candidate an intestinal environment-improving material.
Dependencies are inferred from the claim text.