Methods and arrays for target analyte detection and determination of target analyte concentration in solution

A method of measuring the concentration of a target analyte in a sample using a competitive assay, comprising:

• contacting labelled molecules and target analyte from the sample with a plurality of capture components capable of binding to the labelled molecule or the target analyte, wherein the target analyte competes with the labelled molecules for binding to the capture components, and wherein a captured labelled molecule generates an optical signal and a captured target analyte does not generate an optical signal;
• immobilizing the plurality of capture components with respect to a plurality of reaction vessels comprising the capture components, wherein a portion of the plurality of reaction vessels contains capture components with immobilized labelled molecules, and another portion of the plurality of reaction vessels contains capture components that are not immobilized to labelled molecules;
• determining a total percentage of the reaction vessels that do not contain a captured labelled molecule;
• and
• determining the concentration of the target analyte in the sample from the total percentage.