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US 11,237,170 B2Granted
Styryl phenols, derivatives and their use in methods of analyte detection
AssigneeAAT Bioquest, Inc.
InventorsZhenjun Diwu, Zhen Luo, Jixiang Liu, Pengfei Dong
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Abstract
Styryl compounds useful for preparing biological detections probes, and the use of the Styryl compounds for the detection, discrimination and quantification of biological targets.
Claims16
A method of detecting a target in a sample, wherein the method comprises the steps of:
- (i) incubating a target with one or more binding agents comprising a peroxidase conjugate, wherein the one or more binding agents is/are capable of direct or indirect binding to the target, forming a complex comprising the target and one or more binding agents, wherein at least one binding agent comprises peroxidase activity;
- (ii) incubating the complex of (i) with a peroxide compound and a compound of Formula 1
- wherein,
- R1-R4 are each H;
- R7 and R8 are each H;
- R9 is phenyl;
- X is hydrogen, or a counter cation;
- L is a linker;
- TAG is a biologically detectable moiety connected to R9 via the linker L;
- and m and n are independently an integer from 1 to 100,
- wherein the compound is capable of functioning as a detectable substrate for a peroxidase enzyme or a peroxidase conjugate to provide a deposited compound;
- and
- (iii) detecting the deposited compound, and thereby detecting the target.
10 more elements
A method of detecting a target in a sample, wherein
- the method comprises the steps of:
- (i) incubating a target with one or more binding agents comprising a peroxidase conjugate, wherein the one or more binding agents is/are capable of direct or indirect binding to the target, forming a complex comprising the target and one or more binding agents, wherein at least one binding agent comprises peroxidase activity;
- (ii) incubating the complex of (i) with a peroxide compound of the following Formula
- wherein,
- L is a linker;
- and
- TAG is a biologically detectable moiety;
- wherein the compound is capable of functioning as a detectable substrate for a peroxidase enzyme or a peroxidase conjugate to provide a deposited compound;
- and
- (iii) detecting the deposited compound, and thereby detecting the target.
7 more elements
Dependencies are inferred from the claim text.