Method for identifying modulators of G3BP activity
AssigneeSt. Jude Children's Research Hospital
InventorsJ. Paul Taylor, Peiguo Yang, Wenwei Lin, Taosheng Chen
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Abstract
A method of identifying a lead or candidate compound that modulates the activity of GTPase-Activating Protein SH3 Domain-Binding Proteins (G3BP) is provided, which includes determining whether a compound modulates the interaction between the N-terminal Nuclear Transport Factor 2-like (NTF2L) domain of G3BP and FGDF peptide of ubiquitin specific protease 10 (USP10) or non-structural protein 3 (nsP3).
Claims5
A method of identifying a lead or candidate compound that modulates the activity of GTPase-Activating Protein SH3 Domain-Binding Proteins (G3BP) comprising:
- (a) contacting a test compound in vitro with a N-terminal Nuclear Transport Factor 2-like (NTF2L) domain of G3BP and a peptide containing a FGDF motif for a period of time;
- and
- (b) measuring whether the compound modulates the interaction between the NTF2L domain and the peptide containing a FGDF motif thereby identifying a lead or candidate compound that modulates the activity of G3BP.
A method of identifying a lead or candidate compound that modulates the activity of GTPase-Activating Protein SH3 Domain-Binding Proteins (G3BP) comprising:
- (a) contacting a test compound in vitro with a N-terminal Nuclear Transport Factor 2-like (NTF2L) domain of G3BP and a peptide containing a FGDF motif for a period of time;
- and
- (b) measuring whether the compound modulates the interaction between the NTF2L domain and the peptide containing a FGDF motif thereby identifying a lead or candidate compound that modulates the activity of G3BP,
- wherein the peptide containing a FGDF motif has the amino acid sequence of SEQ ID NO:4 or SEQ ID NO:5.
Dependencies are inferred from the claim text.