Back to results
US 11,237,167 B2Granted
Lp-PLA2 assays and composition with detergent
AssigneeCleveland Heartlab, Inc.
InventorsCelalettin Topbas, Cory Bystrom
Life of this patent
Estimating the term…
Abstract
Provided herein are methods, systems, and compositions for Lp-PLA2 detection assays that employ amounts of detergent to liberate all or nearly all of the Lp-PLA2 molecules from associated lipoprotein particles. In this regard, the true Lp-PLA2 concentration can be detected in a sample, which correlates better with known Lp-PLA2 activity assays.
Claims14
A method of detecting lipoprotein-associated phospholipase A2 (Lp-PLA2) in a sample comprising:
- a) contacting a sample with a first amount of a detergent mix and Lp-PLA2 binding molecules,
- wherein said detergent mix comprises two or more detergents,
- wherein said detergent is not CHAPS,
- wherein said sample comprises Lp-PLA2 associated with lipoprotein particles,
- wherein said first amount of the detergent mix is sufficient such that at least 95% of all of said Lp-PLA2 in said sample that is associated with said lipoprotein particles is liberated from said lipoprotein particles, and
- wherein said Lp-PLA2 binding molecules bind to Lp-PLA2 to form complexes;
- and
- b) detecting said complexes, thereby determining said Lp-PLA2 concentration in said sample.
5 more elements
Dependencies are inferred from the claim text.