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US 11,237,165 B2Granted
Antibody producing non-human animals
AssigneeMerus N.V.
InventorsTon Logtenberg, Mark Throsby, Robert A. Kramer, Rui Daniel Pinto, Cornelis A. de Kruif, Erwin Houtzager
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Abstract
Described are transgenic, non-human animals comprising a nucleic acid encoding an immunoglobulin light chain, whereby the immunoglobulin light chain is a common human, human-like, or humanized light chain. Further provided is methods for producing an immunoglobulin from the transgenic, non-human animal.
Claims14
A method for selecting a single recombinant cell that expresses at least a bispecific antibody, or antibody fragment thereof, the method comprising:
- providing recombinant cells comprising at least two different heavy chain variable regions and a light chain variable region, wherein the at least two different heavy chain variable regions are encoded by nucleic acid sequences obtained from a transgenic non-human animal, and wherein the light chain variable region is able to functionally pair with more than one heavy chain variable region,
- pairing of the variable regions and secretion of the paired variable regions from the recombinant cells resulting in the production of the bispecific antibody,
- and
- selecting a single recombinant cell from the recombinant cells that produces a bispecific antibody that binds two target epitopes,
- wherein the light chain variable region comprises a V and J consisting of germline sequences encoded by a human immunoglobulin light chain V gene segment joined to a human immunoglobulin light chain J gene segment.
2 more elements
Dependencies are inferred from the claim text.