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US 11,237,164 B2Granted
Method for measurement of total protein content and detection of protein via immunoassay in a microfluidic device
AssigneeProteinSimple
InventorsIrina Georgievna Kazakova, Crystal Tran, Jessica Dermody, Annegret Boge, Tom Weisan Yang
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Abstract
Some embodiments described herein relate to systems and methods operable to combine immunoassay and Total Protein techniques in a single sample run. Some embodiments described herein allow for multiple sequential immunoassays to be performed in the same microfluidic device. Some embodiments described herein relate to stripping reagents operable to remove primary antibodies associated with immunoassays. Such stripping reagents can allow for additional immunoassays and/or Total Protein assays to be performed on the same sample.
Claims6
A method, comprising:
- electrophoretically separating a sample containing a plurality of analytes in a microfluidic device;
- immobilizing the plurality of analytes in the microfluidic device;
- introducing a first primary antibody into the microfluidic device, the first primary antibody capable of binding to a first analyte from the plurality of analytes;
- introducing a secondary antibody into the microfluidic device, the secondary antibody capable of binding to the first primary antibody;
- stripping the first primary antibody from the first analyte using a reagent having a pH between 3 and 4.5 and including Tris(2-carboxyethyl)phosphine hydrochloride) (TCEP);
- and
- introducing into the microfluidic device and after stripping the first primary antibody from the first analyte at least one of (i) a second primary antibody capable of binding to a second analyte from the plurality of analytes or (ii) streptavidin capable of non-specifically binding to the plurality of analytes.
4 more elements
Dependencies are inferred from the claim text.