Time-resolved fluorescent immunochromatographic test strip for detecting paclitaxel drugs as well as preparation method and application thereof

A method for detecting paclitaxel drugs by a time-resolved fluorescent immunochromatographic test kit, comprising the steps of:

• (1) pre-treating a sample with a sample diluent to obtain a sample to be tested;
• (2) detecting the sample to be tested by using the time-resolved fluorescent immunochromatographic test kit to obtain a detected test kit;
• and
• (3) analyzing the detected test kit by a fluorescence detection analyzer to obtain a test result;
• wherein:
• the time-resolved fluorescent immunochromatographic test kit comprises a test paper and a sample diluent;
• the test paper comprises a bottom plate and a sample absorption pad;
• a fluorescent microsphere pad, a bonding pad, and an absorbent pad are sequentially overlapped on the bottom plate;
• the fluorescent microsphere pad is sprayed with a fluorescent microsphere-labeled anti-paclitaxel monoclonal antibody;
• a detection area and a quality control area are immobilized on the bonding pad;
• the detection area is sprayed with a paclitaxel hapten-carrier protein conjugate;
• the quality control area is sprayed with a goat anti mouse antibody;
• the anti-paclitaxel monoclonal antibody is prepared by using a paclitaxel hapten-carrier protein conjugate as an immunogen;
• the paclitaxel hapten-carrier protein conjugate is obtained by coupling a 7-xylosyl paclitaxel hapten with a carrier protein;
• and
• the 7-xylosyl paclitaxel has the following formula:
• wherein the anti-paclitaxel monoclonal antibody is expressed from a hybridoma deposited at the China General Microbiological Culture Collection Center (CGMCC) and the CGMCC number is 23027.