Screening methods using olfactory receptors and novel compounds identified using the same
AssigneeMars, Incorporated
InventorsScott McGrane, Matthew Ronald Gibbs
Life of this patent
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Abstract
The presently disclosed subject matter relates to methods of screening raw materials and pet food products to manufacture a palatable pet food product. The presently disclosed subject matter also relates to methods for identifying compounds that modulate the activity and/or expression of an olfactory receptor.
Claims16
A method for identifying a compound capable of modifying palatability of a pet food product, the method comprising:
- a. measuring a first activity of a feline olfactory receptor,
- b. contacting a test agent with the feline olfactory receptor,
- c. measuring a second activity of the feline olfactory receptor after contacting the test agent, and
- d. determining that the test agent is a compound capable of modifying palatability of a pet food product when the second activity of the feline olfactory receptor is increased or decreased compared to the first activity;
- wherein the feline olfactory receptor comprises an amino acid sequence selected from the group consisting of SEQ ID Nos:
- 51-60, wherein the first and second activities are measured by detecting a secondary messenger.
A method for identifying a compound capable of modifying palatability of a pet food product, the method comprising
- a. contacting a test agent with a feline olfactory receptor,
- b. detecting binding between the test agent and one or more amino acids of the feline olfactory receptor, and
- c. determining that the test agent is a compound capable of modifying olfactory profile of a pet food product when the test agent binds one or more amino acids of the-feline olfactory receptor wherein the feline olfactory receptor comprises an amino acid sequence selected from the group consisting of SEQ ID Nos:
- 51-60.
A method for identifying a compound capable of modifying olfactory profile of a pet food product, the method comprising
- a. contacting an olfactory receptor agonist with a feline olfactory receptor,
- b. measure a first activity of the feline olfactory receptor,
- c. contacting a test agent with the feline olfactory receptor,
- d. measure a second activity of the feline olfactory receptor, and
- e. determining that the test agent is a compound capable of modifying palatability of a pet food product when the first activity is lesser than the second activity;
- wherein the feline olfactory receptor comprises an amino acid sequence selected from the group consisting of SEQ ID Nos:
- 51-60, wherein the first and second activities are measured by detecting a secondary messenger.
A method for selecting a pet food product or component thereof, comprising
- a. measuring a first activity of a feline olfactory receptor,
- b. contacting a test agent with the feline olfactory receptor,
- c. measuring a second activity of the feline olfactory receptor after contacting the test agent, and
- d. selecting the test agent as a pet food product, or component thereof, when the second activity of the feline olfactory receptor is increased or decreased compared to the first activity;
- wherein the feline olfactory receptor comprises an amino acid sequence selected from the group consisting of SEQ ID Nos:
- 51-60, wherein the first and second activities are measured by detecting a secondary messenger.
Dependencies are inferred from the claim text.