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US 11,237,138 B2Granted
Selection and design of columns for liquid chromatography
AssigneeThe Wrangler Group
InventorHubert M. Quinn
Life of this patent
Estimating the term…
Abstract
The present disclosure is directed at the selection and design of columns for liquid chromatography including liquid chromatography devices and systems and corresponding methods of operation, particularly in the field of high pressure liquid chromatography (HPLC).
Claims15
A method performed in a liquid chromatography apparatus comprising:
- providing a packed column containing particles having a diameter of about 1.0 μm to 2.0 μm, said column having an internal diameter of about 0.15 cm to 0.20 cm and a column length of about 1 cm to 20 cm;
- eluting a fluid through said column at a volumetric flow rate of 0.10 ml/min to 0.05 ml/min with a solute having a diffusion coefficient in the range of 5.0×10−6 cm2/sec to 5.0×10−5 cm2/sec wherein said column indicates a maximum pressure drop of 1300 bar.
A method for isolating a target molecule comprising:
- providing a packed column containing particles having a diameter of about 1.0 μm to 2.0 μm, said column having an internal diameter of about 0.15 cm to 0.20 cm and a column length of about 1 cm to 20 cm;
- eluting a fluid through said column comprising the target molecule at a volumetric flow rate of 0.10 ml/min to 0.05 ml/min wherein the target molecule has a diffusion coefficient in the range of 5.0×10−6 cm2/sec to 5.0×10−5 cm2/sec wherein said column indicates a maximum pressure drop of 1300 bar;
- and
- recovering portions of said liquid comprising the target molecule.
1 more element
Dependencies are inferred from the claim text.