Fluorescence image analyzing apparatus, image processing method of fluorescence image, and computer program

A cell analysis method comprising:

• capturing first and second fluorescence images of a cell in a sample which is pretreated with first and second fluorescent dyes, the first fluorescence image being a first color based and the second fluorescence image being a second color based;
• extracting one or more first-color bright spots in the first fluorescence image;
• extracting one or more second-color bright spots in the second fluorescence image;
• and
• changing a pixel value of at least either of the first-color bright spots or the second-color bright spots based on a pixel value of the other such that a difference of pixel values between the one of first-color bright spots and one of the second-color bright spots are relatively reduced.

A fluorescence image analyzing apparatus comprising:

• a light source to emit light to a sample pretreated with a fluorescent dye;
• an imaging unit to capture a fluorescence image of a cell irradiated with the light;
• a processing unit that processes the fluorescence image captured by the imaging unit;
• and
• a display unit to display the fluorescence image processed by the processing unit,
• wherein the processing unit performs:
• extracting at least two bright spots in the fluorescence image including a target site;
• changing a pixel value of at least one of the extracted bright spots such that a difference of pixel values between the extracted bright spots are relatively reduced;
• and
• causing the display unit to display the fluorescence image with the pixel value changed.

A fluorescence image analyzing apparatus comprising:

• a light source to emit light to a sample pretreated with a fluorescent dye;
• an imaging unit to capture a fluorescence image of a cell irradiated with the light;
• a processing unit that processes the fluorescence image captured by the imaging unit;
• and
• a display unit to display the fluorescence image processed by the processing unit,
• wherein the processing unit performs:
• capturing first and second fluorescence images of a cell in a sample which is pretreated with first and second fluorescent dyes, the first fluorescence image being a first color based and the second fluorescence image being a second color based;
• extracting one or more first-color bright spots in the first fluorescence image;
• extracting one or more second-color bright spots in the second fluorescence image;
• and
• changing a pixel value of at least either of the first-color bright spots or the second-color bright spots based on a pixel value of the other such that a difference of pixel values between the one of first-color bright spots and one of the second-color bright spots are relatively reduced.