US 11,237,100 B2Granted

Methods using FTIR for plant trait detection and trait introgression

AssigneeCorteva Agriscience LLC

InventorsBala Bhaskar Pudota, Daniel Gachotte, Virginia Stoltz, Terence A. Walsh

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Abstract

Provided are methods and/or systems having advantages of cost effective, time saving, and informative user-friendly characteristics to accomplish trait introgression. The methods provided comprise determining presence of omega-3 fatty acids (for example docosahexaenoic acid or DHA; docosapentaenoic acid or DPA; Alpha linolenic acid or ALA; and eicosapentaenoic acid or EPA) using Fourier Transformed infra Red (FTIR) spectrum. The use of FTIR enables analysis of the oil contained in the seeds using a multivariate-based Mid-FTIR model. The methods and/or systems provided advantages of non-destructive analysis to provide information to facilitate trait introgression and other breeding applications.

Claims5

Dependent claims

A method for determining presence of docosahexaenoic acid (DHA) in a viable whole plant seed, the method comprising

(a) obtaining one or more viable whole plant seeds;
(b) performing a Fourier Transform Infrared (FTIR) spectroscopy analysis of each of the one or more viable whole plant seeds, wherein the spectroscopy analysis comprises use of a detector cooled using liquid nitrogen to detect absorbance at a first absorption band between about 3800 cm−1 wavenumber and about 2600 cm−1 wavenumber;
(c) comparing the absorbance of step (b) to existing reference FTIR absorbance;
(d) selecting at least one of the one or more viable whole plant seeds analyzed in step (b) based on the detected absorbance of step (b), wherein each selected seed is viable and predicted to contain DHA based on the detected absorbance of step (b);
and
(e) planting or breeding with the at least one seed selected in step (d).

3 more elements

Dependencies are inferred from the claim text.

Facts

Application: 15/601,157Filed 22 May 2017 · us provisional application
Priority: 9 Jun 2016
Published: US 2017/0356019 A114 Dec 2017
Granted: 1 Feb 2022
Term ends: Not estimatedThe source records don’t support an estimate.

Classification10 codes

G01N 21/35
Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry:…
A23L 33/105
Using additives: plant extracts, their artificial duplicates or their derivatives
A23L 33/12
Fatty acids or derivatives thereof; Fats or oils: fatty acids or derivatives thereof
G01N 21/3563
Using infrared light: for analysing solids; Preparation of samples therefor