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US 11,237,096 B2Granted
Cell capture system and method of use
AssigneeBio-Rad Laboratories, Inc.
InventorKalyan Handique
Life of this patent
Estimating the term…
Abstract
A cell capture system including an array, an inlet manifold, and an outlet manifold. The array includes a plurality of parallel pores, each pore including a chamber and a pore channel, an inlet channel fluidly connected to the chambers of the pores; an outlet channel fluidly connected to the pore channels of the pores. The inlet manifold is fluidly connected to the inlet channel, and the outlet channel is fluidly connected to the outlet channel. A cell removal tool is also disclosed, wherein the cell removal tool is configured to remove a captured cell from a pore chamber.
Claims20
A system comprising:
- a substrate defining a set of chambers, the set of chambers configured to retain and partition material of a sample and each of the set of chambers comprising:
- one or more walls defining a chamber volume, and
- an open surface permitting access of sample material to be partitioned, into the chamber volume, from a direction perpendicular to the broad surface of the substrate,
- a layer sealing the set of chambers, wherein the layer supports optical interrogation by an imaging platform, wherein each chamber of the set of chambers is individually optically accessible from a direction perpendicular to the broad surface of the substrate, through the layer;
- an inlet channel positioned upstream of the set of chambers and fluidly coupled to the set of chambers, wherein the inlet channel has a first end and a second end and is fluidly sealed at the second end;
- and
- an outlet channel positioned downstream of the set of chambers and fluidly coupled to the set of chambers, wherein fluid from the inlet channel reaches the outlet channel only by passing into the set of chambers.
4 more elements
A method comprising:
- providing a substrate comprising an inlet channel, an outlet channel, and a set of chambers in fluid communication with the inlet channel and the outlet channel, wherein flow from the inlet channel is configured to reach the outlet channel only upon passing into the set of chambers, and wherein the inlet channel has a first end and a second end and is fluidly sealed at the second end;
- receiving a fluid sample, comprising material to be partitioned, into the inlet channel;
- capturing and partitioning said material, by way of the set of chambers;
- and
- providing an environment for conducting a set of processes at the substrate, wherein the set of processes comprises an imaging process configured for imaging contents of the set of chambers through a layer sealing the set of chambers.
2 more elements
Dependencies are inferred from the claim text.