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US 11,236,382 B2Granted
Method and system for sample preparation
AssigneeIntegrated Nano-Technologies, Inc.
InventorsDennis M. Connolly, Tara Holz, Vera Tannous, Christopher Kilcoin, Konstantin Aptekarev, David B. Bailey, Richard S. Murante, Nathaniel E. Wescott
Life of this patent
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Abstract
A method for preparing a sample by utilizing a shearing force in the presence of a size stabilizer to break apart the sample to obtain nucleic acid molecules in a usable size range. Once nucleic acid molecules are obtained, magnetic nanoparticles are used to concentrate and clean the nucleic acid molecules for further testing.
Claims20
A disposable cartridge for preparing an assay fluid, comprising:
- a cartridge body including (i) a cylindrical surface defining a fixed port and (ii) a syringe barrel defining a bore for receiving a moveable plunger, the fixed port disposed in fluid communication with the bore of the syringe barrel;
- and
- a rotor mounting to the cartridge body and including a surface slideably engaging the cylindrical surface of the cartridge body along a mating interface, the rotor including:
- a plurality of chambers each configured to facilitate at least one operation on the assay fluid, and
- a plurality of moveable ports disposed along the surface of the rotor and in fluid communication with at least one of the plurality of chambers;
- wherein the rotor is rotationally indexed about an axis parallel to the cylindrical surface such that the plurality of moveable ports are selectively aligned with the fixed port of the cartridge body.
3 more elements
A method for preparing an assay fluid, including the steps of:
- configuring a cartridge body with:
- (i) a cylindrical surface defining a fixed port and (ii) a syringe barrel defining a bore for receiving a moveable plunger, the fixed port disposed in fluid communication with the bore of the syringe barrel;
- configuring a rotor for mounting to the cartridge body and including (i) a cylindrical surface for slideably engaging the cylindrical surface of the cartridge body along a mating interface, (ii) a plurality of chambers, and (iii) a plurality of movable ports disposed in the cylindrical surface and in fluid communications with the plurality of chambers;
- rotationally indexing the rotor about an axis parallel to the cylindrical surface of the cartridge body;
- and
- displacing the moveable plunger in the syringe barrel to cause the assay fluid to translate from the fixed port to one or more of the moveable ports, the assay fluid being moved from the chambers of the rotor to a reaction chamber where properties of the assay fluid are evaluated.
3 more elements
Dependencies are inferred from the claim text.