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US 11,236,377 B2Granted
Compositions and methods related to nucleic acid synthesis
AssigneeNuclera Nucleics Ltd.
InventorsMichael C. Chen, Radu A. Lazar, Jiahao Huang, Gordon R. McInroy
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Abstract
The invention relates to the use of specific terminal deoxynucleotidyl transferase (TdT) enzymes in a method of nucleic acid synthesis, to methods of synthesizing nucleic acids, and to the use of kits comprising said enzymes in a method of nucleic acid synthesis. The invention also relates to the use of terminal deoxynucleotidyl transferases and 3′-blocked nucleotide triphosphates in a method of template independent nucleic acid synthesis.
Claims17
A method of nucleic acid synthesis, which comprises:
- (a) providing an initiator oligonucleotide immobilized on a solid support;
- and
- (b) adding a 3′-blocked nucleoside triphosphate to said initiator in the presence of a terminal deoxynucleotidyl transferase (TdT) comprising an amino acid sequence having at least 95% sequence identity to any one of SEQ ID NOS:
- 1 to 5 and 8, or a functional fragment thereof having terminal deoxynucleotidyl transferase activity, and comprising an N-terminal or a C-terminal truncation.
1 more element
Dependencies are inferred from the claim text.