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US 11,236,376 B2Granted
Alpha (1,2) fucosyltransferases suitable for use in the production of fucosylated oligosaccharides
AssigneeGlycosyn LLC
InventorsMatthew Ian Heidtman, Massimo Merighi, John M. McCoy
Life of this patent
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Abstract
The invention provides compositions and methods for engineering E. coli or other host production bacterial strains to produce fucosylated oligosaccharides, and the use thereof in the prevention or treatment of infection.
Claims26
A method for producing a fucosylated oligosaccharide in a host bacterium comprising
- providing said host bacterium comprising a genetic modification to reduce level of β-galactosidase activity, a defective colanic acid synthesis pathway, a mutation in an ATP-dependent intracellular protease, a mutation in a thyA gene, and an exogenous lactose-accepting α(1,2) fucosyltransferase gene from a genus consisting of Bacteroides;
- culturing said host bacterium in the presence of lactose;
- and
- retrieving the fucosylated oligosaccharide from said host bacterium or from a culture supernatant of said host bacterium, wherein less than 1% (w/w) of the level of the fucosylated oligosaccharide produced by said host bacterium is from 3-fucosyllactose (3-FL).
1 more element
A method for producing a fucosylated oligosaccharide in a host bacterium comprising
- providing said host bacterium comprising a reduced level of β-galactosidase activity, a defective colanic acid synthesis pathway, a mutation in an ATP-dependent intracellular protease, a mutation in a thyA gene, and an exogenous lactose-accepting α(1,2) fucosyltransferase gene;
- culturing said host bacterium in the presence of lactose;
- and
- retrieving a fucosylated oligosaccharide from said host bacterium or from a culture supernatant of said host bacterium, wherein said exogenous lactose-accepting α(1,2) fucosyltransferase gene is from a bacterial strain consisting of Bacteroides vulgatus.
1 more element
Dependencies are inferred from the claim text.