Lipid production
AssigneeEvonik Operations GmbH
InventorsMirja Wessel, Steffen Schaffer, Anne Jeremias, Martin Schilling, Hans Henning Wenk
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Abstract
The present invention relates to at least one cell for producing at least one lipid with general formula II from at least one carbon substrate, wherein R1 and R2 independently of one another comprises identical or different organic radicals each with 5 to 13 carbon atoms, wherein the cell is a non-pathogenic cell that is genetically modified to increase the heterologous expression relative to the wild type cell of: an enzyme (E2) capable of converting 3-hydroxyalkanoyl-3-hydroxyalkanoyl-CoA/ACP or 3-(3-hydroxyalkanoyloxy)alkanoic acid (HAA) and NDP-glucose into β-D-glucopyranosyl-3-hydroxyalkanoyl-3-hydroxyalkanoate.
Claims18
A microbial cell for producing at least one lipid with general formula II from at least one carbon substrate,
- wherein R1 and R2 independently of one another comprise identical or different organic radicals each with 5 to 13 carbon atoms;
- and
- wherein the cell is a non-pathogenic cell that is genetically modified to increase the heterologous expression relative to the wild type cell of an enzyme (E2) capable of converting 3-hydroxyalkanoyl-3-hydroxyalkanoyl-CoA/ACP and/or 3-(3-hydroxy-alkanoyloxy)alkanoic acid (HAA) in combination with NDP-glucose into β-D-glucopyranosyl-3-hydroxyalkanoyl-3-hydroxyalkanoate wherein the enzyme E2 is a glycosyltransferase (EC 2.4) comprising SEQ ID NO:
- 4 or variant thereof, wherein the variant comprises at least 90% sequence identity to SEQ ID NO:
- 4.
Dependencies are inferred from the claim text.