Fatty acid decarboxylase and its uses
AssigneesCommissariat A L'energie Atomique Et Aux Energies Alternatives, Centre National De La Recherche Scientifique, Universite D'Aix-Marseille
InventorsFrederic Beisson, Damien Sorigue, Bertrand Legeret, Stephan Cuine, Stephanie Blangy, Gilles Peltier
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Abstract
The present invention relates to the identification of a new class of fatty acid decarboxylases and its uses, in particular for producing alkanes/alkenes from fatty acids.
Claims18
A method for producing alkanes and/or alkenes from fatty acids comprising contacting a polypeptide having fatty acid decarboxylase activity, comprising a sequence having at least 80% sequence identity with one of SEQ ID NOs:
- 1-3 or 5-8 with fatty acids having from 12 to 18 carbon atoms in length and in the presence of light having a wavelength between 300 and 540 nm, the polypeptide also comprising a FAD binding domain and the consensus sequence G-X-L-(X)4-C-[D/E]-X-G-[A/G]-F-X-[K/R] (SEQ ID NO:
- 4), X being any amino acid, said fatty acids being optionally substituted with at least one hydroxyl group or methyl group.
A method for producing alkanes and/or alkenes from fatty acids, wherein a recombinant host cell expressing a polypeptide having at least 80% identity with one of SEQ ID NOs:
- 1, 2, 3, 5, 6, 7 or 8, the polypeptide also comprising a FAD binding domain and the consensus sequence G-X-L-(X)4-C-[D/E]-X-G-[A/G]-F-X-[K/R] (SEQ ID NO:
- 4), X being any amino acid, is cultured and alkanes and/or alkenes are recovered.
A method for producing alkanes and/or alkenes from fatty acids comprising contacting a polypeptide having fatty acid decarboxylase activity with fatty acids and light with a wavelength between 300 and 540 nm, wherein the polypeptide having fatty acid decarboxylase comprises a sequence having at least 85% identity with a sequence selected from the group consisting of SEQ ID NO:
- 1, SEQ ID NO:
- 2, SEQ ID NO:
- 3, SEQ ID NO:
- 5, SEQ ID NO:
- 6, SEQ ID NO:
- 7, and SEQ ID NO:
- 8 and wherein the fatty acids and the corresponding decarboxylated alkanes and/or alkenes have from 12 to 18 carbon atoms in length, said fatty acids being optionally substituted with at least one hydroxyl group or methyl group.
Dependencies are inferred from the claim text.