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US 11,236,366 B2Granted
Production of squalene from hyper-producing yeasts
AssigneeNovartis AG
InventorMichael Broeker
Life of this patent
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Abstract
A method for preparing purified yeast is disclosed, where the squalene source is a yeast that hyper-produces squalene. The squalene is useful for pharmaceutical purposes. For instance, it can be used to prepare an oil-in-water emulsion, and the emulsion is particularly suitable for use as an immunological adjuvant.
Claims19
A process for preparing an oil-in-water emulsion for parenteral use in humans, comprising the steps of:
- (a) providing a yeast culture that comprises a controlled medium free from animal-origin products cultured under conditions so that the yeast comprises at least 2% cell dry weight squalene;
- (b) purifying the squalene from the culture for parenteral use in humans such that it is of a pharmaceutical grade;
- and (c) combining the squalene purified in step (b) with an aqueous component to form the oil-in-water emulsion for parenteral use in humans, wherein the yeast is a modified strain (1) that expresses a truncated 3 hydroxy-3-methylglutaryl-CoA reductase (HMGR) enzyme that has a cytosolic location, and/or (2) that, relative to an unmodified parent strain, hyper-expresses HMGR.
A process for preparing an oil-in-water emulsion for parenteral use in humans, comprising the steps of:
- (a) obtaining squalene that has been purified for parenteral use in humans such that it is of a pharmaceutical grade from a yeast culture that comprises a controlled medium free from animal-origin products cultured under conditions so that the yeast comprises at least 2% by dry weight squalene;
- and (b) combining the squalene purified of step (a) with an aqueous component to form the oil-in-water emulsion for parenteral use in humans, wherein the yeast is a modified strain (1) that expresses a truncated 3 hydroxy-3- methylglutaryl-CoA reductase (HMGR) enzyme that has a cytosolic location, and/or (2) that, relative to an unmodified parent strain, hyper-expresses HMGR.
Dependencies are inferred from the claim text.