Hybrid proteins and uses thereof
AssigneeSymvivo Corporation
InventorsHerbert Alexander Graves, Mark Andrew Fox
Life of this patent
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Abstract
There are disclosed hybrid proteins comprising at least one signal sequence; at least one DNA binding domain; and at least one cell penetrating peptide (CPP) domain. In embodiments the CPP domain is a TAT domain, and the DNA binding domain is a HU domain. There is also disclosed the use of the hybrid proteins to introduce exogenous DNA into target cells, and methods for introducing exogenous DNA into target cells using the hybrid proteins.
Claims10
A method of transforming a Bifidobacteria sp. bacterium with a DNA plasmid, the method comprising contacting the Bifidobacteria sp. bacterium with a hybrid protein in complex with the DNA plasmid, the hybrid protein comprising:
- (i) at least one Bifidobacteria signal peptide selected from the group consisting of an alpha amylase signal peptide and an alpha arabinosidase signal peptide;
- (ii) at least one DNA binding domain which binds to the DNA plasmid, the at least one DNA binding domain positioned C-terminal in the hybrid protein relative to the at least one Bifidobacteria signal peptide;
- and
- (iii) at least one cell penetrating peptide (CPP) domain positioned C-terminal in the hybrid protein relative to the at least one DNA binding domain.
Dependencies are inferred from the claim text.