Wheat stem rust resistance genes and methods of use
AssigneeCommonwealth Scientific and Industrial Research Organisation
InventorsEvans Lagudah, Sambasivam Periyannan, Burkhard Steuernagel, Kamil Witek, Brande Wulff
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Abstract
Compositions and methods and for enhancing the resistance of wheat plants to wheat stem rust caused by Puccinia graminis f. sp. tritici are provided. The compositions comprise nucleic acid molecules encoding resistance (R) gene products and variants thereof and plants, seeds, and plant cells comprising such nucleic acid molecules. The methods for enhancing the resistance of a wheat plant to wheat stem rust comprise introducing a nucleic acid molecule encoding an R gene product into a wheat plant cell. Additionally provided are methods for using the wheat plants in agriculture to limit wheat stem rust.
Claims9
A transgenic plant or seed comprising stably incorporated in its genome a heterologous polynucleotide comprising a nucleotide sequence selected from the group consisting of:
- (a) the nucleotide sequence set forth in SEQ ID NO:
- 1, 4, 7, 19, 22, or 25;
- (b) a nucleotide sequence encoding the amino acid sequence set forth in SEQ ID NO:
- 2, 5, 8, 20, 23, or 26;
- (c) the nucleotide sequence set forth in SEQ ID NO:
- 3, 6, 9, 21, 24, or 27;
- and
- (d) a nucleotide sequence encoding an amino acid sequence having at least 96% sequence identity to the amino acid sequence set forth in SEQ ID NO:
- 2, wherein the nucleic acid molecule is capable of conferring resistance to stem rust to a wheat plant comprising the nucleic acid molecule.
A method of limiting wheat stem rust in agricultural crop production, the method comprising planting a wheat seed and growing a wheat plant under conditions favorable to the growth and development of the wheat plant, wherein the wheat seed comprises stably incorporated in its genome a heterologous polynucleotide comprising a nucleotide sequence selected from the group consisting of:
- (a) the nucleotide sequence set forth in SEQ ID NO:
- 1, 4, 7, 19, 22, or 25;
- (b) a nucleotide sequence encoding the amino acid sequence set forth in SEQ ID NO:
- 2, 5, 8, 20, 23, or 26;
- (c) the nucleotide sequence set forth in SEQ ID NO:
- 3, 6, 9, 21, 24, or 27;
- and
- (d) a nucleotide sequence encoding an amino acid sequence having at least 96% sequence identity to the amino acid sequence set forth in SEQ ID NO:
- 2, wherein the nucleic acid molecule is capable of conferring resistance to stem rust to a wheat plant comprising the nucleic acid molecule.
Dependencies are inferred from the claim text.