Production of DHA and other LC PUFAs in plants
AssigneesDow AgroSciences LLC, Dsm IP Assets B.V.
InventorsTerence A. Walsh, Daniel Gachotte, Ann Owens Merlo, Dayakar Reddy Pareddy, James Metz, Scott Bevan, Jerry Kuner, Paul Gordon Roessler
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Abstract
The invention provides recombinant host organisms genetically modified with a polyunsaturated fatty acid (PUFA) synthase system and one or more accessory proteins that allow for and/or improve the production of PUFAs in the host organism. The present invention also relates to methods of making and using such organisms as well as products obtained from such organisms.
Claims21
A method to produce an oil comprising docosahexaenoic acid (C22:6, n-3) (DHA) and docosapentaenoic acid (C22:5, n-6) (DPA(n-6)), the method comprising:
- Recovering the oil from a genetically modified soybean seed comprising:
- a first polynucleotide operably linked to a seed-specific promoter, the first polynucleotide encoding a polyunsaturated fatty acid (PUFA) synthase polypeptide comprising an amino acid sequence that is at least 95% identical to SEQ ID NO:1;
- a second polynucleotide operably linked to a seed-specific promoter, the second polynucleotide encoding a PUFA synthase polypeptide comprising an amino acid sequence that is at least 95% identical to SEQ ID NO:2;
- a third polynucleotide operably linked to a seed-specific promoter, the third polynucleotide encoding a PUFA synthase system polypeptide comprising an amino acid sequence that is at least 95% identical to SEQ ID NO:3;
- a fourth polynucleotide operably linked to a seed-specific promoter, the fourth polynucleotide encoding a phosphopantetheinyl transferase (PPTase) polypeptide comprising an amino acid sequence that is at least 95% identical to SEQ ID NO:5;
- and
- a fifth polynucleotide operably linked to a seed-specific promoter, the fifth polynucleotide encoding an acyl-CoA synthase (ACoAS) comprising an amino acid sequence that is at least 95% identical to SEQ ID NO:4.
Dependencies are inferred from the claim text.