Method for producing dextran polymer-based, amplified nucleic acid aptamer nanoconstruct selectively capturing target molecule

A method for producing dextran polymer-based amplified nucleic acid aptamer nanoconstructs which selectively capture a target molecule, the method comprising the steps of:

• (a) linking an aptamer sequence (Apt) for the target molecule and the DNA sequence of SEQ ID NO:
• 1 to a dextran polymer, thereby producing a dextran polymer-based DNA aptamer (Dex-DNA-Apt);
• (b) linking an aptamer sequence (Apt) for the target molecule and a sequence complementary to the DNA sequence to the dextran polymer, thereby producing a dextran polymer-based cDNA aptamer (Dex-cDNA-Apt) having a dextran polymer-based cDNA sequence;
• (c) mixing the Dex-DNA-Apt produced in step (a) with the Dex-cDNA-Apt produced in step (b), followed by annealing, thereby producing nanoconstructs (NCs);
• and
• (d) subjecting the nanoconstructs to rolling circle amplification to amplify the aptamer (Apt), thereby producing nanoconstructs (aNCs) having an amplified aptamer Apt for the target molecule.