Cell sorting
AssigneesThe Broad Institute, Inc., Massachusetts Institute of Technology
InventorsSilvana Konermann, David Feldman, Feng Zhang, Paul Blainey
Life of this patent
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Abstract
The invention provides for use of CRISPR-Cas systems to sort barcoded cells or molecules. Cells or nucleic acid molecules may be sorted from a heterogenous population by targeting a barcode of interest specific for a cell or cell progeny or nucleic acid molecule of interest.
Claims8
A method of recovering one or more cells of interest from a heterogeneous population of cells, wherein the heterogenous population of cells are obtained by a method comprising:
- i. delivering a plurality of reporter constructs to the heterogeneous population of cells,
- wherein each reporter construct comprises a unique barcode, and a selection marker or reporter under transcriptional control of a promoter, and
- wherein one reporter construct is integrated into the genome of each cell, whereby the reporter construct is passed to its progeny upon propagation of the population of cells;
- ii. expanding the heterogeneous population of cells for one or more generations, wherein each cell and the progeny of each cell in the heterogeneous population of cells have the same reporter construct, and
- iii. splitting the heterogeneous population of cells into two or more subpopulations;
- a. said method comprising delivering to a subpopulation of the expanded heterogeneous population of cells a CRISPR-Cas enzyme and one or more guide RNAs targeting one or more barcodes specific for one or more cells of interest, wherein said targeting activates transcription of the selection marker or reporter in the one or more cells of interest;
- and
- b. separating cells that express said selection marker or reporter from the remaining cells within the heterogeneous population that do not express said selection marker or reporter.
A method of recovering one or more cells of interest from a heterogeneous population of cells, wherein the heterogenous population of cells are obtained by a method comprising:
- i. delivering a plurality of reporter constructs to the heterogeneous population of cells,
- wherein each reporter construct comprises a unique barcode, and a selection marker or reporter under transcriptional control of a promoter, and
- wherein one reporter construct is integrated into the genome of each cell, whereby the reporter construct is passed to its progeny upon propagation of the population of cells;
- ii. expanding the heterogeneous population of cells for one or more generations, wherein each cell and the progeny of each cell in the heterogeneous population of cells have the same reporter construct, and
- iii. splitting the heterogeneous population of cells into two or more subpopulations;
- a. said method comprising delivering to a subpopulation of the expanded heterogeneous population of cells a CRISPR-Cas enzyme and one or more guide RNAs targeting one or more barcodes specific for one or more cells of interest, wherein said CRISPR-Cas enzyme creates an insertion or deletion in the barcode in the one or more cells of interest that causes a frameshift within an open reading frame of the selection marker or reporter, thereby activating or inactivating the selection marker or reporter;
- and
- b. separating cells that express said selection marker or reporter from the remaining cells within the heterogeneous population that do not express said selection marker or reporter.
Dependencies are inferred from the claim text.