Isolation of stem cells from adipose tissue by ultrasonic cavitation, and methods of use
AssigneeCell-Innovations IP Pty Ltd
InventorsRalph Bright, Pelin Bright, Bruce Hansen, Wayne Thomas
Life of this patent
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Abstract
In one embodiment, the present invention relates to a non-enzymatic method for isolating stem cells from adipose tissue, wherein the method comprises treating adipose tissue with ultrasonic cavitation to break up the adipose tissue and lyses mature adipocytes, resulting in a stromal vascular fraction containing viable stromal/stem cells.
Claims9
A method of isolating a stromal vascular fraction comprising viable stromal/stem cells from adipose tissue, the method comprising treating the adipose tissue with ultrasonic cavitation about 90 seconds to about 8 minutes with an amplitude and cycle corresponding to that of a Hielscher UP200S ultrasonic device set at an amplitude of about 20% to about 70%, and a cycle of about 0.2 to about 0.8 seconds;
- wherein in said method, the amplitude does not exceed 70%, the cycle does not exceed 0.8 seconds, the duration of cavitation does not exceed 8 minutes, and the temperature of the adipose tissue does not exceed about 43° C. to about 45° C.
Dependencies are inferred from the claim text.