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US 11,236,318 B2Granted
Recombinant Factor C and method for producing the same, and method for measuring endotoxin
AssigneeSeikagaku Corporation
InventorsHikaru Mizumura, Toshio Oda, Shun-ichiro Kawabata
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Abstract
A horseshoe crab Factor C protein having activity of Factor C, wherein the horseshoe crab is selected from Tachypleus tridentatus, Limulus polyphemus, and Carcinoscorpius rotundicauda, and wherein the horseshoe crab Factor C protein is produced through being recombinantly expressed from a Chinese Hamster Ovary (CHO) DG44 cell or HEK cell.
Claims21
A horseshoe crab Factor C protein having activity of Factor C,
- wherein the horseshoe crab is selected from the group consisting of Tachypleus tridentatus, and Limulus polyphemus, and wherein the horseshoe crab Factor C protein is produced through being recombinantly expressed from a Chinese Hamster Ovary (CHO) DG44 cell or Human Embryonic Kidney (HEK) 293 cell, and
- wherein the Factor C protein contains (α-2,3)-linked terminal sialic acid in a greater amount, as compared with a corresponding native horseshoe crab Factor C and/or a corresponding Factor C protein recombinantly expressed using Sf9 as a host cell.
A Factor C protein having activity of Factor C,
- wherein the Factor C protein is produced through being recombinantly expressed from a Chinese Hamster Ovary (CHO) DG44 cell or Human Embryonic Kidney (HEK) 293 cell, and
- wherein the expressed Factor C protein comprises an amino acid sequence selected from the group consisting of:
- (A) the amino acid sequence of SEQ ID NO:
- 2, and the Factor C protein contains (α-2,3)-linked terminal sialic acid in a greater amount, as compared with a native horseshoe crab Factor C of Tachypleus tridentatus and/or a corresponding Factor C protein recombinantly expressed using Sf9 as a host cell;
- and
- (B) an amino acid sequence having 90% or higher sequence identity to the amino acid sequence of SEQ ID NO:2, and having activity of Factor C, and the Factor C protein contains (α-2,3)-linked terminal sialic acid in a greater amount, as compared with a native horseshoe crab Factor C of Tachypleus tridentatus and/or a corresponding Factor C protein recombinantly expressed using Sf9 as a host cell;
- and
- wherein the Factor C protein exhibits a residual activity of at least one selected from the group consisting of:
- (1) 10% or higher in the presence of 21 mM sodium citrate;
- and
- (2) 15% or higher in the presence of 16 mM magnesium sulfate.
8 more elements
A horseshoe crab Factor C protein having activity of Factor C,
- wherein the horseshoe crab is Limulus polyphemus, and wherein the horseshoe crab Factor C protein is produced through being recombinantly expressed from a Human Embryonic Kidney (HEK) 293 cell, and
- wherein the Factor C protein exhibits a residual activity of at least one selected from the group consisting of:
- (1) 10% or higher in the presence of 21 mM sodium citrate;
- and
- (2) 15% or higher in the presence of 16 mM magnesium sulfate.
2 more elements
Dependencies are inferred from the claim text.