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US 11,236,315 B2Granted
Thermophile peptidoglycan hydrolase fusion proteins and uses thereof
AssigneesThe United States of America as represented by the Secretary of Agriculture, University of Maryland
InventorsDavid M. Donovan, Steven M. Swift
Life of this patent
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Abstract
The disclosure relates to chimeric recombinant lysins comprising at least one thermophile endolysin catalytic domain and at least one cell wall binding domain. Also disclosed are polynucleotides encoding the chimeric recombinant lysins, host cells expressing the chimeric recombinant lysins, and use of such chimeric recombinant lysins.
Claims30
A polynucleotide encoding a chimeric recombinant lysin, comprising:
- [I] a first nucleic acid molecule encoding at least one thermophile endolysin catalytic domain from:
- [i] PlyGspY412 as set forth in SEQ ID NO:
- 4, or a variant thereof having at least 90% sequence identity to SEQ ID NO:
- 4;
- [ii] PlyGspY4 as set forth in SEQ ID NO:
- 16, or a variant thereof having at least 90% sequence identity to SEQ ID NO:
- 16;
- or
- [iii] PlyGve2 as set forth in SEQ ID NO:
- 23, or a variant thereof having at least 90% sequence identity to SEQ ID NO:
- 23;
- [II] a second nucleic acid molecule encoding at least one cell wall binding domain from C. perfringens endolysins:
- [a] PlyCP10 as set forth in SEQ ID NO:
- 5, or a variant thereof having at least 90% sequence identity to SEQ ID NO:
- 5;
- [b] PlyCP18 as set forth in SEQ ID NO:
- 6, or a variant thereof having at least 90% sequence identity to SEQ ID NO:
- 6;
- [c] PlyCP33 as set forth in SEQ ID NO:
- 7, or a variant thereof having at least 90% sequence identity to SEQ ID NO:
- 7;
- [d] PlyCP41 as set forth in SEQ ID NO:
- 8, or a variant thereof having at least 90% sequence identity to SEQ ID NO:
- 8;
- or
- [e] PlyCP26 as set forth in SEQ ID NO:
- 9, or a variant thereof having at least 90% sequence identity to SEQ ID NO:
- 9;
- [III] optionally a third nucleic acid molecule encoding a linker between the thermophile endolysin catalytic domain and the cell wall binding domain;
- and
- [IV] optionally a fourth nucleic acid molecule encoding a polyhistidine tag, wherein the chimeric recombinant lysin is not a fusion of PlyGve2 and PlyCP26.
28 more elements
A chimeric recombinant lysin polypeptide comprising:
- [I] at least one thermophile endolysin catalytic domain from:
- [i] PlyGspY412 as set forth in SEQ ID NO:
- 4, or a variant thereof having at least 90% sequence identity to SEQ ID NO:
- 4;
- [ii] PlyGspY4 as set forth in SEQ ID NO:
- 16, or a variant thereof having at least 90% sequence identity to SEQ ID NO:
- 16;
- or
- [iii] PlyGve2 as set forth in SEQ ID NO:
- 23, or a variant thereof having at least 90% sequence identity to SEQ ID NO:
- 23;
- [II] at least one cell wall binding domain from C. perfringens endolysins:
- [a] PlyCP10 as set forth in SEQ ID NO:
- 5, or a variant thereof having at least 90% sequence identity to SEQ ID NO:
- 5;
- [b] PlyCP18 as set forth in SEQ ID NO:
- 6, or a variant thereof having at least 90% sequence identity to SEQ ID NO:
- 6;
- [c] PlyCP33 as set forth in SEQ ID NO:
- 7, or a variant thereof having at least 90% sequence identity to SEQ ID NO:
- 7;
- [d] PlyCP41 as set forth in SEQ ID NO:
- 8, or a variant thereof having at least 90% sequence identity to SEQ ID NO:
- 8;
- or
- [e] PlyCP26 as set forth in SEQ ID NO:
- 9, or a variant thereof having at least 90% sequence identity to SEQ ID NO:
- 9;
- [III] optionally a third nucleic acid molecule encoding a linker between the thermophile endolysin catalytic domain and the cell wall binding domain;
- and
- [IV] optionally a fourth nucleic acid molecule encoding a polyhistidine tag,
- wherein the chimeric recombinant lysin polypeptide is not a fusion of PlyGve2 and PlyCP26.
29 more elements
Dependencies are inferred from the claim text.