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AssigneeCodexis, Inc.
InventorsMathew G. Miller, Chinping Chng, Oscar Alvizo, Melissa Ann Mayo, James Nicholas Riggins, Xiang Yi, Jonathan S. Penfield, Gjalt W. Huisman, Jared Davis, Yasushi Saotome
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The present invention provides engineered RNA polymerase variants and compositions comprising these variants. The present invention further provides engineered T7 RNA polymerase variants and compositions comprising these variants. These variants have been evolved for selective incorporation of the m7G(5′)ppp(5′)m7G cap analog over GTP at the initiation of in vitro transcription. The present invention also provides methods for selective capping of RNA transcripts.
A method for producing capped mRNA, comprising performing an in vitro transcription reaction utilizing an engineered RNA polymerase, wherein said engineered RNA polymerase comprises a polypeptide sequence having at least 95% or more sequence identity to the reference sequence of SEQ ID NO:4, wherein said engineered RNA polymerase comprises substitution in said polypeptide sequence at position 664, and wherein the amino acid positions of said polypeptide sequence are numbered with reference to SEQ ID NO:4.
Dependencies are inferred from the claim text.