Recombinant vector and method for producing reconstituted cytochrome P450 oxygenase-reductase fusion protein using the same
AssigneeIndustry Foundation of Chonnam National University
InventorsGeun Joong Kim, Chul Ho Yun, Dae Eun Cheong, Su Kyoung Yoo, Hye Ji Choi, Thien Kim Le
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Abstract
A recombinant vector according to an embodiment of the present invention may be used for producing a reconstituted cytochrome P450 oxygenase-reductase fusion protein. Oxygenase and reductase may be independently expressed in a host cell into which the recombinant vector is introduced, and then may be fused with a split intein so that it is possible to increase the heme content contained in the active site of the oxygenase and improve enzyme activity and stability.
Claims11
A recombinant vector for producing a cytochrome P450 oxygenase-reductase fusion protein in which cytochrome P450 oxygenase and reductase for the same are independently expressed and reconstituted, the recombinant vector comprising:
- a first polynucleotide which encodes the cytochrome P450 oxygenase;
- a second polynucleotide which encodes the reductase;
- and a third polynucleotide which is interposed between the first and second polynucleotides and encodes a split intein.
Dependencies are inferred from the claim text.