Method for differentiating induced pluripotent stem cells, which are prepared from endocardium-derived adult stem cells, into cardiovascular cells, and use thereof

A method of preparing induced pluripotent stem cells, the method comprising:

• producing endocardium-derived adult stem cells by:
• suspending and seeding peripheral blood mononuclear cells (PBMCs) isolated from peripheral blood in an endothelial cell growth medium (EGM),
• removing T cells, and
• culturing the resulting cells while replacing the medium every day for 5 days to 8 days;
• introducing, into the endocardium-derived adult stem cells, a gender-determining region Y (SRY)-box 2 (SOX2) gene, a v-myc avian myelocytomatosis viral oncogene homolog (c-MYC) gene, an octamer-binding transcription factor 4 (OCT4) gene, and a Kruppel-like factor 4 (KLF4) gene to provide gene-introduced cells;
• producing epithelial-like cells by culturing the gene-introduced cells in an endothelial cell growth medium for 8 days to 12 days while replacing the medium every day;
• mounting the epithelial-like cells on feeder cells and culturing the mounted cells in the endothelial cell growth medium for 3 days to 7 days;
• and
• subsequently continuing to culture the resulting cells while replacing the endothelial cell growth medium with an embryonic stem cell (ES) medium, which provides induced pluripotent stem cells.