Microfluidic device for controlling the geometry of living bodies

A microfluidic device to manipulate, select, treat, or cultivate living bodies, comprising:

• a first chamber;
• a second chamber;
• a network of guiding tracks located between the first chamber and the second chamber, the network of guiding tracks comprising a first guiding track extending between and connecting the first chamber and the second chamber and a second guiding track extending between and connecting the first guiding track with at least two interconnections, the second guiding track comprising a curved part, the curved part exhibiting a concavity facing the second chamber or a part of the network of guiding tracks connected to the second chamber, and
• repellent areas or repellent volumes located between the first chamber and the second chamber and defining the network of guiding tracks, the repellent areas or repellent volumes having an affinity to support growth or adhesion of living bodies that is less than an affinity of the network of guiding tracks and of the first and second chambers to support the growth or adhesion of living bodies,
• wherein the network of guiding tracks defined by the repellent areas or repellent volumes is shaped such that the network of guiding tracks is asymmetrical relative to a lateral axis extending perpendicular to a longitudinal axis of the microfluidic device, the longitudinal axis extending between the first chamber and the second chamber, the lateral axis located intermediately along a longitudinal dimension of the network of guiding tracks;
• and
• wherein at least one of the repellent areas or repellent volumes comprises a first side in contact with or facing the second chamber and a second side in contact with or facing the first chamber, wherein:
• the first side has at least one cusp having a tip angle, and
• the second side has no or fewer cusps than the first side and/or the second side has at least one cusp, the at least one cusp of the second side having a tip angle that is greater than the tip angle of the at least one cusp of the first side.