Method of producing cell culture chip

A method of producing a cell culture, the method comprising:

• a step A of obtaining a cell culture flow path substrate including a recessed portion on which one or more cell culture flow paths are formed and a flat portion around the recessed portion, the flat portion being a portion which is bonded to a cell culture flow path top plate;
• a step B of selectively disposing a first adhesive layer and a second adhesive layer on a first substrate having transparency to obtain the cell culture flow path top plate;
• and
• a step C of thermocompression-bonding the cell culture flow path substrate to the cell culture flow path top plate to obtain a microflow path structure having a hollow structure to produce the cell culture chip including the microflow path structure in which the cell culture flow path substrate on which the cell culture flow path is formed is bonded to the cell culture flow path top plate through the first and second adhesive layers;
• wherein in the step B, the first adhesive layer is disposed in an area which faces the recessed portion when the cell culture flow path top plate is bonded to the cell culture flow path substrate,
• the second adhesive layer is disposed in an area which faces the flat portion face when the cell culture flow path top plate is bonded to the cell culture flow path substrate,
• the first adhesive layer is formed using a first adhesive containing a polyester-based resin having a glass transition temperature of 5° C. or higher, and
• the second adhesive layer is formed using a second adhesive containing a polyester-based resin having a glass transition temperature of lower than 5° C.