Manufacturing process for polysaccharide beads
AssigneeCytiva BioProcess R&D AB
InventorsPer Erik Emilsson, Susanna Klara Margareta Lindberg, Jonny Wernersson, Jonas Gustafsson, Adam Hurynowicz
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Abstract
The invention discloses a method of manufacturing polysaccharide beads, comprising the steps of: i) providing a water phase comprising an aqueous solution of a polysaccharide; ii) providing an oil phase comprising at least one water-immiscible organic solvent and at least one oil-soluble emulsifier; iii) emulsifying the water phase in the oil phase to form a water-in-oil (w/o) emulsion; and iv) inducing solidification of the water phase in the w/o emulsion, wherein the organic solvent is an aliphatic or alicyclic ketone or ether.
Claims21
A method of manufacturing polysaccharide beads, comprising the steps of:
- i) providing a water phase comprising an aqueous solution of a polysaccharide and an alkali, wherein the alkali is present in the water phase at a concentration of 0.1 M to 2 M;
- ii) providing an oil phase comprising at least one water-immiscible organic solvent and at least one oil-soluble emulsifier;
- iii) emulsifying said water phase in said oil phase to form a water-in-oil (w/o) emulsion;
- and
- iv) inducing solidification of said water phase in said w/o emulsion to form substantially spherical polysaccharide beads,
- wherein said at least one organic solvent is an alicyclic ketone or an ether.
A method of manufacturing polysaccharide beads, comprising the steps of:
- i) providing a water phase comprising an aqueous solution of a polysaccharide and an alkali, wherein the alkali is present in the water phase at a concentration of 0.1 M to 2 M;
- ii) providing an oil phase comprising at least one water-immiscible organic solvent and at least one oil-soluble emulsifier;
- iii) emulsifying said water phase in said oil phase to form a water-in-oil (w/o) emulsion;
- and
- iv) inducing solidification of said water phase in said w/o emulsion to form substantially spherical polysaccharide beads,
- wherein said at least one organic solvent is an alicyclic ketone or an ether, does not contain halogens, and has Hansen solubility parameter values in the ranges of δD=15.0-18.5 MPa1/2, δP=3.5-8.5 MPa1/2 and δH=4.0-5.5 MPa1/2, or wherein said oil phase comprises a mixture of halogen-free water-immiscible organic solvents, said mixture having Hansen solubility parameter values in the ranges of δD=15.0-18.5 MPa1/2, δP=3.5-8.5 MPa1/2 and δH=4.0-5.5 MPa1/2.
Dependencies are inferred from the claim text.