US 11,236,149 B2 · Microbe-binding molecules and uses thereof

Abstract

Described herein are engineered microbe-targeting molecules, microbe-targeting articles, kits comprising the same, and uses thereof. Such microbe-targeting molecules, microbe-targeting articles, or the kits comprising the same can not only bind or capture of a microbe or microbial matter thereof, but they also have improved capability (e.g., enhanced sensitivity or signal intensity) of detecting a microbe or microbial matter. Thus, the microbe-targeting molecules, microbe-targeting articles, and/or the kit described herein can be used in various applications, e.g., but not limited to assays for detection of a microbe or microbial matter, diagnostic and/or therapeutic agents for diagnosis and/or treatment of an infection caused by microbes in a subject or any environmental surface, and/or devices for removal of a microbe or microbial matter from a fluid.

Claims20

Dependent claims

A method of capturing or removing a microbe or microbial matter in a sample, comprising:

a) contacting the sample with a carrier scaffold linked to a microbe-binding molecule comprising:
i) a collagen domain;
ii) an Fc domain of an immunoglobulin;
and
iii) a microbe-binding domain comprising a helical domain and a carbohydrate recognition domain (CRD);
A) wherein the Fc domain links the collagen domain to the microbe-binding domain;
B) wherein the collagen domain links the Fc domain to the microbe-binding domain, and wherein the microbe-binding molecule excludes a cysteine-rich crosslinking domain;
or
C) wherein the microbe-binding domain links the collagen domain to the Fc domain, and wherein the microbe-binding molecule excludes a cysteine-rich crosslinking domain;
and
b) removing the sample that is not bound to the microbe-binding molecule.

8 more elements

A method of detecting a microbe or microbial matter in a sample, comprising:

a) contacting the sample with a carrier scaffold linked to a first microbe-binding molecule comprising:
i) a collagen domain;
ii) an Fc domain of an immunoglobulin;
and
iii) a microbe-binding domain comprising a helical domain and a carbohydrate recognition domain (CRD);
A) wherein the Fc domain links the collagen domain to the microbe-binding domain;
B) wherein the collagen domain links the Fc domain to the microbe-binding domain, and wherein the microbe-binding molecule excludes a cysteine-rich crosslinking domain;
or
C) wherein the microbe-binding domain links the collagen domain to the Fc domain, and wherein the microbe-binding molecule excludes a cysteine-rich crosslinking domain;
b) removing the sample that is not bound to the first microbe-binding molecule;
c) contacting the sample with a second microbe-binding molecule that is conjugated to a detectable label;
and
d) detecting the detectable label.

10 more elements

Dependencies are inferred from the claim text.

Facts

Application

16/879,015Filed 20 May 2020 · division

Priority

6 Aug 2015

Published

US 2020/0283506 A110 Sep 2020

Granted

1 Feb 2022

Term ends

Not estimatedThe source records don’t support an estimate.

Classification12 codes

C07K 14/78
From animals; from humans: connective tissue peptides, e.g. collagen, elastin, laminin, fibronectin, vitronectin or cold insoluble globulin [CIG]
C07K 14/00
Peptides having more than 20 amino acids
C07K 14/4726
Not used: lectins
C07K 19/00
Hybrid peptides , i.e. peptides covalently bound to nucleic acids, or non-covalently

Family

Application62/201,745Us provisional application · filed 6 Aug 2015

PCT/US2016/045509Parent of this continuation · filed 4 Aug 2016

Application16/553,635Parent of this division · filed 28 Aug 2019

US 10,435,457 B2Parent of this continuation · filed 8 Oct 2019

US 2020/0283506 A1Pre-grant publication · published 10 Sep 2020

US 10,696,733 B2Parent of this division

Application15/750,788Parent of this continuation

Application16/553,635Parent of this continuation

US 2018/0371058 A1Published application

US 2019/0389935 A1Published application

US 11,236,149 B2This patent

From the US filing history. Foreign family members aren’t listed.

Microbe-binding molecules and uses thereof

Life of this patent

Microbe-binding molecules and uses thereof

Life of this patent

Abstract

Claims20