Mixed bed ion exchange adsorber
AssigneeEMD Millipore Corporation
InventorsMatthew T. Stone, John P. Amara
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Abstract
The present invention refers to new species of an ion exchange adsorber which is suitable for the separation of host cell proteins (HCPs), antibody fragments and low molecular weight substances from solutions containing antibodies. The invention especially refers to a process for purifying biological samples by separating biomolecules of interest and impurities, comprising steps of contacting a sample with said chromatography media consisting of fibers, said fibers having imparted thereon functionality enabling ion exchange chromatography and/or hydrophobic interaction.
Claims5
A single housing comprising a packed bed of fibers for purification of biomolecules, said packed bed having a blend of a first chromatography media and a second chromatography media, said first chromatography media comprising fibers having surface pendant functional groups enabling anion-exchange chromatography, and said second chromatography media comprising fibers having surface pendant functional groups enabling cation-exchange or hydrophobic interaction chromatography, or said first chromatography media comprising fibers having surface pendant functional groups enabling cation-exchange chromatography, and said second chromatography media comprising fibers having surface pendant functional groups enabling hydrophobic interaction chromatography.
Dependencies are inferred from the claim text.