Method and composition for hydrolyzing eggshell membrane
AssigneeEggnovo S.L.
InventorsManuel A. La Nuez García, Andrés Aguirre González
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Abstract
The present invention relates to a method for hydrolyzing eggshell membrane, comprising the step of treating a suitable amount of eggshell in a solution containing a denaturing agent, a reducing agent, a buffer, and an enzyme. The invention also relates to a composition for hydrolyzing eggshell membrane according to the preceding method.
Claims5
A method for hydrolyzing eggshell membrane, comprising the step of treating eggshell membrane in a solution having a pH in the range between 6 and 7, and comprising a denaturing agent, a reducing agent, a buffer, and an enzyme, wherein the denaturing agent is sodium lauryl sulfate (SDS), the reducing agent is selected from the group consisting of sodium hydroxymethanesulfinate, sodium metabisulfite, and dithiothreitol (DTT), and the enzyme is an endopeptidase selected from the group of cysteine proteases.
Dependencies are inferred from the claim text.