Back to results
US 11,235,972 B2Granted
Sequence design for efficient assembly of nucleic acid structures
AssigneePresident and Fellows of Harvard College
InventorsNikhil Gopalkrishnan, Thomas E. Schaus, Peng Yin
Life of this patent
Estimating the term…
Abstract
The present disclosure provides, in some aspects, methods and compositions for producing nucleic acid nanostructures having little to no kinetic barriers to self-assembly.
Claims14
A nucleic acid nanostructure comprising:
- (a) a deoxyribonucleic acid (DNA) strand having a length of at least 100 nucleotides and having a nucleotide sequence that comprises less than 10% of one of C, G, T or A nucleotides;
- and
- (b) 10-2000 oligonucleotides, wherein each oligonucleotide is at least partially complementary to and at least partially bound to the DNA strand.
A nucleic acid nanostructure of comprising:
- (a) a deoxyribonucleic acid (DNA) strand having a length of at least 100 nucleotides and having a nucleotide sequence that comprises less than 10% of one of C, G, T or A nucleotides;
- and
- (b) a plurality of oligonucleotides, wherein each oligonucleotide is at least partially complementary to and at least partially bound to the DNA strand, wherein the nanostructure is a three-dimensional DNA origami structure and has a diameter of 10-500 nm.
A kit comprising
- (a) a deoxyribonucleic acid (DNA) strand having a length of at least 100 nucleotides and having a nucleotide sequence that comprises less than 10% of one of C, G, T or A nucleotides;
- and
- (b) 10-2000 oligonucleotides, wherein each oligonucleotide is at least partially complementary to and at least partially bound to the DNA strand.
A method of producing a DNA origami nanostructure, comprising
- incubating, in a reaction vessel,
- (a) a deoxyribonucleic acid (DNA) strand having a length of at least 100 nucleotides and having a nucleotide sequence that comprises less than 10% of one of C, G, T or A nucleotides, and
- (b) a plurality of oligonucleotides, wherein each oligonucleotide is at least partially complementary to the DNA strand,
- under conditions that result in binding of the oligonucleotides to the DNA strand to form a DNA origami nanostructure.
1 more element
Dependencies are inferred from the claim text.