System and method of using multi-chambered receptacles

A method of processing a sample in a receptacle comprising a plurality of chambers, wherein each of the chambers is connected to at least one other chamber by a portal and at least a first one of the chambers is formed of a flexible material, the method comprising:

• A. causing gas bubbles contained in the first chamber to accumulate in a portion of the first chamber;
• B. applying a compressive external force to the first chamber to cause some or all liquid contents of the first chamber to flow into an interconnected second chamber through a portal connecting the first and second chambers;
• and
• C. preventing the gas bubbles accumulated in a portion of the first chamber from flowing through the portal into the second chamber during step B;
• wherein the gas bubbles are prevented from flowing through the portal into the second chamber during step B by a wall extending from a peripheral boundary of the first chamber into an interior space of the first chamber.