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US 11,235,286 B2Granted
Organic solvent method for preparing membrane protein based nanosheets and membranes based on nanosheets
AssigneeThe Penn State Research Foundation
InventorsManish Kumar, Tingwei Ren, Woochul Song, Yu-Ming Tu
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Abstract
The present disclosure describes compositions and methods for preparing membrane protein nanosheets and two-dimensional crystals. In particular, the methods employ a solvent. A mixture of a polymer and a membrane protein is solubilized in the solvent, applied to a substrate, and subsequently dried to form the nanosheet or two-dimensional crystal. Applicants have surprisingly found that the membrane proteins maintain their structure when exposed to solvents during the short processing time utilized.
Claims19
A method of preparing a nanosheet or two-dimensional crystal comprising:
- preparing a mixture comprising a polymer and a membrane protein comprising a pore or channel structure, wherein said polymer is solubilized in an organic solvent and the membrane protein is added to the polymer solubilized in the organic solvent to form the mixture;
- applying said mixture to a substrate;
- removing said solvent to form a dried nanosheet and/or two-dimensional crystal, wherein the pore or channel structure of the membrane protein is maintained in the nanosheet and/or two-dimensional crystal;
- and
- rehydrating said dried nanosheet and/or two-dimensional crystal in a rehydration buffer and water;
- wherein the rehydrating separates the nanosheet and/or two-dimensional crystal from the substrate.
3 more elements
Dependencies are inferred from the claim text.