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US 11,234,446 B2Granted
Stabilization of chymosin by polymers
AssigneeChr. Hansen A/S
InventorsMartin Lund, Jonas Jacobsen, Johannes Maarten Van Den Brink
Life of this patent
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Abstract
A liquid or dried granulated milk clotting aspartic protease enzyme composition and process for isolating a milk clotting aspartic protease enzyme of interest.
Claims8
A dried granulated milk clotting aspartic protease enzyme composition comprising:
- (i) a milk clotting aspartic protease enzyme at a strength of from 25 International Milk-Clotting Units (IMCU)/g of the composition to 30000 IMCU/g of the composition, as determined by a relative milk-clotting activity test (REMCAT) using the milk-clotting activity of the international reference standard for rennet as a reference standard;
- (ii) a polymer at a concentration of from 1 ppm to 5000 ppm (w/w), and
- (iii) a salt;
- wherein the polymer has the following characteristics (a), (b) and (c):
- (a) the polymer is a polymer of at least one monomer selected from ethylene oxide, vinylpolypyrrolidone, vinyl alcohol, vinyl acetate, acrylonitrile, acrylate and methacrylate;
- (b) the polymer has a molecular mass of from 200 g/mol to 50000 g/mol;
- and
- (c) the polymer has a number of monomers (“n number”) of from n=5 to n=1250;
- and
- wherein a suspension of the dried granulated composition in water has a pH of from 2 to 8.
7 more elements
Dependencies are inferred from the claim text.