Technologies for nucleotide sequence screening

A method for assessing threat levels associated with synthesizing one or more nucleotide sequences, the method comprising:

• comparing, with a compute engine, each nucleotide sequence of the one or more nucleotide sequences to each of a plurality of reference sequences stored in a reference database;
• detecting, with the compute engine, whether hazardous content is present in each nucleotide sequence of the one or more nucleotide sequences based upon the comparison of that nucleotide sequence to each of the plurality of reference sequences, including selecting a plurality of nucleotide sequence segments of the one or more nucleotide sequences that each matches part of one of the plurality of reference sequences, in response to a determination that a matching length between each selected nucleotide sequence segment and the corresponding partial reference sequence does not satisfy a threshold length, combining the selected plurality of nucleotide sequence segments into a composite nucleotide sequence, and applying a sliding window to the composite nucleotide sequence to analyze a matching percentage between each portion of the composite nucleotide sequence having the threshold length and the one of the plurality of reference sequences;
• assigning, with the compute engine one of a plurality of threat levels to synthesizing each nucleotide sequence based upon the detection of whether hazardous content is present in that nucleotide sequence;
• determining that a threat level assigned to a nucleotide sequence of the one or more nucleotide sequences does not represent a threat;
• and
• synthesizing, after determining that the threat level does not represent a threat, the nucleotide sequence of the one or more nucleotide sequences.

A system for assessing threat levels associated with synthesizing nucleotide sequences, the system comprising:

• a server to communicate with a remote frontend over a network to receive, from the frontend, a request to screen a plurality of nucleotide sequences to be synthesized for hazardous content and to report, to the frontend, a result of screening the plurality of nucleotide sequences to be synthesized for hazardous content, wherein the frontend is to provide a graphical user interface to allow a user to input the plurality of nucleotide sequences to be screened for hazardous content by uploading a single file containing the plurality of nucleotide sequences and to display to the user the result of screening the plurality of nucleotide sequences for hazardous content;
• and
• a processor to:
• compare each nucleotide sequence of the plurality of nucleotide sequences to each of a plurality of reference sequences stored in a reference database, wherein the plurality of nucleotide sequences includes at least a thousand nucleotide sequences;
• detect whether hazardous content is present in each nucleotide sequence of the plurality of nucleotide sequences based upon the comparison of that nucleotide sequence to each of the plurality of reference sequences, including selecting a plurality of nucleotide sequence segments of the plurality of nucleotide sequences that each matches part of one of the plurality of reference sequences, in response to a determination that a matching length between each selected nucleotide sequence segment and the corresponding partial reference sequence does not satisfy a threshold length, combining the selected plurality of nucleotide sequence segments into a composite nucleotide sequence, and applying a sliding window to the composite nucleotide sequence to analyze a matching percentage between each portion of the composite nucleotide sequence having the threshold length and the one of the plurality of reference sequences, and
• assign one of a plurality of threat levels to synthesizing each nucleotide sequence based upon the detection of whether hazardous content is present in that nucleotide sequence;
• wherein the result reported to the frontend by the server comprises the threat level assigned to synthesizing each nucleotide sequence of the plurality of nucleotide sequences.