Immunoassay for simultaneously screening anti-platelet antibodies and performing platelets cross matching and test apparatus for carrying out same

An immunoassay for simultaneously screening anti-platelet antibodies and performing platelet cross matching, comprising the steps of:

• forming a plurality of first mixtures b reacting a combination reagent of a plurality of different platelet antigens with a first portion of a serum sample from a subject;
• simultaneously forming a plurality of second mixtures by reacting a plurality of randomly selected platelet samples with a second portion of the serum sample from the subject;
• wherein in each of the first and second mixtures there are a plurality of immunity compounds formed by combining the platelet antigens with the anti-platelet antibodies in the serum of the subject, and other platelet antigens and other antibodies in the serum of the subject not forming the immunity compounds;
• preparing an interception device including a plurality of receptacles and a filter net with the receptacles placed thereon, the filter net having a plurality of apertures having a size less than 4.0 μm;
• placing each of the first and second mixtures in one of the receptacles;
• washing the first and second mixtures wherein the first and second mixtures forming the immunity compounds are intercepted by the filter net and the other antibodies and the other platelet antigens in the serum of the subject not forming the immunity compounds pass through the filter net;
• adding at least one signal sensing reagent to each of the receptacles;
• reacting the signal sensing reagent with the intercepted first and second mixtures forming the immunity compounds to form a plurality of final products;
• and
• performing a signal sensing to determine both whether the final products contain anti-platelet antibodies and further simultaneously determine compatibility of cross matching of respective platelet samples,
• wherein the washing is implemented by gravitational penetration, capillary action, vacuum suction or plunger pressing.