Method for the determination of antibiotic susceptibility through stimulated Raman metabolic imaging
OwnerJi-xin Cheng, Mohamed Seleem +1inventors
InventorsJi-xin Cheng, Mohamed Seleem, Weili Hong
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Abstract
A method for the determination of antibiotic susceptibility through stimulated Raman scattering microscopy is disclosed. The method utilizes a imaging apparatus adapted to collect a laser signal through a sample having a bacteria for imaging the metabolism of the sample. The sample can be manipulated with an antibiotic for imaging to determine the susceptibility of a bacteria to the provided antibiotic.
Claims12
A method for the determination of the antibiotic susceptibility of a bacterium, the method comprising:
- preparing a bacteria immobilization gel;
- cultivating the bacteria in a medium having a nutrient source;
- adding a preselected antibiotic to the medium to form a sample;
- incubating the antibiotic and bacteria within the medium for a preselected period of time;
- centrifuging the sample;
- washing the sample;
- depositing the sample on the bacteria immobilization gel;
- collecting an image of the sample on the bacteria immobilization gel, through coherent Raman microscopy.
A method for rapid determination of antibiotic susceptibility in bacteria using microscopy, comprising:
- cultivating the bacteria in a medium having a nutrient source;
- adding a preselected antibiotic having a pre-selected concentration to the medium to form a sample;
- incubating the sample within the medium for a preselected period of time;
- centrifuging the sample;
- washing the sample;
- depositing the sample on a bacteria immobilization gel;
- imaging the sample on the bacteria immobilization gel, using coherent Raman microscopy.
Dependencies are inferred from the claim text.