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US 11,231,355 B2Granted
Cell capture system and method of use
AssigneeBio-Rad Laboratories, Inc.
InventorKalyan Handique
Life of this patent
Estimating the term…
Abstract
A cell capture system including an array, an inlet manifold, and an outlet manifold. The array includes a plurality of parallel pores, each pore including a chamber and a pore channel, an inlet channel fluidly connected to the chambers of the pores; an outlet channel fluidly connected to the pore channels of the pores. The inlet manifold is fluidly connected to the inlet channel, and the outlet channel is fluidly connected to the outlet channel. A cell removal tool is also disclosed, wherein the cell removal tool is configured to remove a captured cell from a pore chamber.
Claims20
A method comprising:
- providing a substrate comprising an inlet channel, an outlet channel, a set of fluid distribution channels connected to the inlet channel, a set of fluid receiving channels connected to the outlet channel, and a set of compartments in fluid communication with a fluid distribution channel of the set of fluid distribution channels and a fluid receiving channel of the set of fluid receiving channels, the fluid distribution channel paired with the fluid receiving channel, wherein the inlet channel has a first end and a second end and is fluidly sealed at the second end, and wherein flow from the inlet channel is configured to reach the outlet channel only upon passing into the set of compartments;
- capturing and partitioning a set of targets of a fluid sample, by way of the set of compartments;
- and
- providing an environment for conducting a set of processes at the substrate, wherein the set of processes comprises an amplification reaction and an optical detection process configured for detecting signals derived from the set of targets of the set of compartments.
1 more element
A method comprising:
- providing a substrate comprising an inlet channel, an outlet channel, and a set of compartments in fluid communication with the inlet channel and the outlet channel, wherein the inlet channel has a first end and a second end and is fluidly sealed at the second end, and wherein flow from the inlet channel is configured to reach the outlet channel only upon passing into the set of compartments;
- capturing and partitioning a set of targets of a fluid sample, by way of the set of compartments, wherein the set of targets comprise material associated with cancer cells;
- and
- providing an environment for conducting a set of processes at the substrate, wherein the set of processes comprises an optical detection process configured for detecting signals derived from the set of targets of the set of compartments.
1 more element
Dependencies are inferred from the claim text.