An intelligent system for preparing cryo-electron microscopy samples, comprising a control center, an ultra-low temperature liquid tank, a sample holding mechanism, a sample processing mechanism, a position adjustment mechanism and a sample transfer mechanism, wherein the ultra-low temperature liquid tank, the sample holding mechanism, the sample processing mechanism, the position adjustment mechanism and the sample transfer mechanism are signally connected to the control center;
- the ultra-low temperature liquid tank comprises a liquid tank body for holding liquid nitrogen to provide an ultra-low temperature environment required for samples;
- the liquid tank body comprises a bottom wall and a side wall, and the bottom wall and the side wall constitute a chamber with an upward opening;
- the bottom wall comprises a first bottom wall and a second bottom wall, wherein the first bottom wall is provided with a first mounting portion for installing the sample holding mechanism, and the second bottom wall is provided with a second mounting portion matched with the sample transfer mechanism;
- a partition is arranged between the first bottom wall and the second bottom wall, wherein the partition and the first bottom wall constitute a first chamber, and the partition and the second bottom wall constitute a second chamber;
- the sample holding mechanism is configured to limit a position of a to-be-processed sample;
- the sample transfer mechanism is configured to transfer a processed sample in a preset environment;
- the position adjustment mechanism is configured to adjust horizontal and vertical positions of the sample processing mechanism;
- the sample processing mechanism is configured to cut or polish the to-be-processed sample;
- and
- in a working process, the control center controls the ultra-low temperature liquid tank to provide a preset temperature environment based on a target sample type, activates the position adjustment mechanism based on position information of the sample holding mechanism in the first chamber to drive the sample processing mechanism to perform processing according to a preset processing route, and activates, based on information about the processed sample to be transferred into the second chamber, the sample transfer mechanism to transfer the processed sample in the preset environment.