Nano-porous anodic aluminum oxide membrane for healthcare and biotechnology

A method of manufacturing a nano-structured aluminum oxide film, comprising:

• degreasing an aluminum plate using a degreasing solution;
• electropolishing the aluminum plate after degreasing with an electropolishing solution that is free of cytotoxic chemicals;
• pre-anodizing the aluminum plate after electropolishing by immersing the aluminum plate in an anodization acid solution for a first predetermined time period of between five and twenty minutes to form a protective film on the aluminum plate and then lifting the aluminum plate partially out of the anodization acid solution so that a first portion of the aluminum plate having the protective film is exposed and a second portion of the aluminum plate remains immersed in the anodization acid solution;
• anodizing the second portion of the aluminum plate after pre-anodizing with the anodization acid solution for a second predetermined time period that is longer than the first predetermined time period to form an anodized membrane on the second portion of the aluminum plate;
• separating the anodized membrane from the aluminum plate without using cytotoxic chemicals so that the anodized membrane is detached from the aluminum plate and the first portion of the aluminum plate intact remains intact;
• and
• cleaning the anodized membrane.