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US 11,230,730 B2Granted
Nucleotide reagent solutions
AssigneeLife Technologies Corporation
InventorsJamsheed Ghadiri, Karta Atehortua-Khalsa
Life of this patent
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Abstract
A reagent solution includes water, a nucleotide, and tris(2-carboxyethyl)phosphine in a range of 0.5 μM to 1000 μM. The reagent solution can further include a non-ionic surfactant in an amount of 0.001% to 1% or a biocidal agent in an amount of 0.001% to 1%. The reagent solution can include salts, such as sodium chloride or magnesium sulfate.
Claims8
A reagent solution consisting essentially of:
- water;
- a nucleotide in the range of 100 nanomolar to 500 μM;
- tris(2-carboxyethyl)phosphine in a range of 0.5 μM to 10 μM;
- a non-ionic surfactant in a range of 0.001% to 0.1%;
- magnesium sulfate in an amount of 0.001 M to 0.1 M;
- sodium chloride in an amount of 0.01 M to 0.5 M;
- and
- a biocidal agent.
5 more elements
Dependencies are inferred from the claim text.