Production of a flavour compound in a host cell
AssigneeAxxence Aromatic GmbH
InventorsKatarina Cankar, Martinus Julius Beekwilder, Hendrik Jan Bosch, Peter Hans van der Schaft, Wilhelmina Maria Johanna Weemen
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Abstract
The present invention relates to the field of biotechnology; specifically the production of a flavor compound (raspberry ketone) in a host cell.
Claims10
A Corynebacterium glutarnicum cell expressing a functional heterologous enzyme with tyrosine ammonium lyase (TAL) activity and further expressing at least one functional enzyme selected from the group consisting of a 4-coumarate-CoA ligase (4CL) and a benzalacetone synthase (BAS) and optionally further expressing a heterologous benzalacetone reductase (BAR), wherein the cell produces at least 5 mg/L raspberry ketone when cultured in the absence of p-coumaric acid.
The cell according to claiml, wherein the polynucleotide sequence encoding at least one of said enzymes is codon optimized.
Dependencies are inferred from the claim text.