Bivalent antibodies masked by coiled coils
AssigneeSeagen Inc.
InventorsVivian Trang, Matthew R. Levengood, Peter Senter
Life of this patent
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Abstract
The invention provides bivalent antibodies including two light and heavy chain pairs. The N-termini of one or both light and heavy chain pairs are linked via linkers comprising a protease cleavage site to coiled-coil forming peptides that associate to form a coiled coil reducing binding affinity of at least one light-heavy chain pair to a target.
Claims20
A bivalent antibody comprising two light and heavy chain pairs, wherein the N-termini of the light and heavy chains of at least one of the pairs are linked, via linkers comprising a protease cleavage site, to coiled-coil forming peptides that associate to form a coiled coil reducing binding affinity of the antibody to a target relative to the binding affinity of the antibody in naked form;
- wherein (a) a peptide comprising the sequence QGASTSVDELQAEVDQLEDENYALKTKVAQLRKKVEKLGSIPVSLRSG (SEQ ID NO:
- 34) provides the linker comprising the protease cleavage site and the coiled-coil forming peptide linked to the light chain and a peptide comprising the sequence QGASTTVAQLEEKVKTLRAENYELKSEVQRLEEQVAQLGSIPVSLRSG (SEQ ID NO:
- 31) provides the linker comprising the protease cleavage site and the coiled-coil forming peptide linked to the heavy chain;
- or (b) a peptide comprising the sequence QGASTTVAQLEEKVKTLRAENYELKSEVQRLEEQVAQLGSIPVSLRSG (SEQ ID NO:
- 31) provides the linker comprising the protease cleavage site and the coiled-coil forming peptide linked to the light chain and a peptide comprising the sequence QGASTSVDELQAEVDQLEDENYALKTKVAQLRKKVEKLGSIPVSLRSG (SEQ ID NO:
- 34) provides the linker comprising the protease cleavage site and the coiled-coil forming peptide linked to the heavy chain.
Dependencies are inferred from the claim text.