Exosome production method
AssigneesToagosei Co., Ltd., University Public Corporation Osaka
InventorsNahoko Baileykobayashi, Tetsuhiko Yoshida, Ikuhiko Nakase
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Abstract
The method of producing induced lightweight exosomes (iLE) provided by the present invention includes preparing a cell culture containing prescribed cultured cells; supplying at least once an artificially produced synthetic peptide to the cell culture and culturing the cell culture for a prescribed interval; and obtaining exosomes produced by the cultured cells following culturing. Here, the synthetic peptide is provided with: (1) an exosome-inducing peptide sequence and (2) a CPP sequence, and has a total number of amino acid residues of 100 or less.
Claims7
A method of producing induced lightweight exosomes (iLE), comprising:
- preparing a cell culture containing exosome-producing cells;
- adding at least once an artificially produced synthetic peptide to the cell culture containing exosome-producing cells;
- culturing the exosome-producing cells in the presence of the artificially produced synthetic peptide;
- and obtaining exosomes produced by the exosome-producing cells from the cell culture following the culturing;
- wherein the artificially produced synthetic peptide comprises:
- (1) an exosome-inducing peptide consisting of the amino acid sequence of SEQ ID NO:
- 1;
- and,
- (2) a cell penetrating peptide (CPP);
- and wherein the artificially produced synthetic peptide is no more than 100 amino acids in length.
Dependencies are inferred from the claim text.